Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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PEKÁROVÁ Blanka KLUMPLER Tomáš TŘÍSKOVÁ Olga HORÁK Jakub JANSEN Séverine DOPITOVÁ Radka BORKOVCOVÁ Petra PAPOUŠKOVÁ Veronika NEJEDLÁ Eliška SKLENÁŘ Vladimír MAREK Jaromír ŽÍDEK Lukáš HEJÁTKO Jan JANDA Lubomír

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj The Plant Journal
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2011.04637.x/abstract;jsessionid=B5B70BA3083D8EC46EA118AB8CD89B26.d03t02?systemMessage=Wiley+Online+Library+will+be+disrupted+5+Nov+from+10-12+GMT+for+monthly+maintenance
Doi http://dx.doi.org/10.1111/j.1365-313X.2011.04637.x
Obor Genetika a molekulární biologie
Klíčová slova multistep phosphorelay; receiver domain; CKI1; crystal structure; NMR spectroscopy; Arabidopsis thaliana
Popis Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co-factor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF3 ) on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3.
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