Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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DVOŘÁK Pavel BURÝŠKOVÁ Barbora POPELÁŘOVÁ Barbora EBERT Birgitta E. BOTKA Tibor BUJDOŠ Dalimil SÁNCHEZ-PASCUALA Alberto SCHÖTTLER Hannah HAYEN Heiko DE LORENZO Víctor BLANK Lars M. BENEŠÍK Martin

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj Nature Communications
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.nature.com/articles/s41467-024-46812-9
Doi http://dx.doi.org/10.1038/s41467-024-46812-9
Klíčová slova Applied microbiology; Bacterial evolution; Experimental evolution; Metabolic engineering
Přiložené soubory
Popis To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by rational metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous xylose isomerase pathway remained unresolved. Here, we elucidate the xylose metabolism and establish a foundation for further engineering followed by ALE. First, native glycolysis is derepressed by deleting the local transcriptional regulator gene hexR. We then enhance the pentose phosphate pathway by implanting exogenous transketolase and transaldolase into two lag-shortened strains and allow ALE to finetune the rewired metabolism. Subsequent multilevel analysis and reverse engineering provide detailed insights into the parallel paths of bacterial adaptation to the non-native carbon source, highlighting the enhanced expression of transaldolase and xylose isomerase along with derepressed glycolysis as key events during the process.
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