Evolution of plant telomerase RNAs: farther to the past, deeper to the roots

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

FAJKUS Petr KILAR Agata Magdalena NELSON A.D.L. HOLA M. PESKA V. GOFFOVÁ Ivana FOJTOVÁ Miloslava ZACHOVÁ Dagmar FULNEČKOVÁ Jana FAJKUS Jiří

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Nucleic acids research
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://watermark.silverchair.com/gkab545.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAtYwggLSBgkqhkiG9w0BBwagggLDMIICvwIBADCCArgGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMJH7i4repl5kssFDwAgEQgIICic2a0sBtSCxRB0q2AJY0_-lH8kpqQpRwLwLA4PxPLpLSwC
Doi http://dx.doi.org/10.1093/nar/gkab545
Klíčová slova TEMPLATE-BOUNDARY DEFINITION; SECONDARY STRUCTURE; POLYMERASE-III; SNRNA GENES; SEQUENCE; IDENTIFICATION; PROMOTER; PSEUDOKNOT; ELEMENT; ARABIDOPSIS
Popis The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs. linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomere repeats. [GRAPHICS] .
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.