Distribution of SARS-CoV-2 Lineages in the Czech Republic, Analysis of Data from the First Year of the Pandemic

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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KLEMPT Petr BRZON Ondrej KASNY Martin KVAPILOVA Katerina HUBACEK Petr BRIKSI Ales BEZDÍČEK Matěj KOUDELAKOVA Vladimira LENGEROVÁ Martina HAJDUCH Marian DREVINEK Pavel POSPÍŠILOVÁ Šárka KRIEGOVA Eva MACEK Milan KVAPIL Petr

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Microorganisms
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.mdpi.com/2076-2607/9/8/1671
Doi http://dx.doi.org/10.3390/microorganisms9081671
Klíčová slova SARS-CoV-2; metagenomics; variants; phylogeny; massively parallel sequencing
Popis In the Czech Republic, the current pandemic led to over 1.67 million SARS-CoV-2- positive cases since the recording of the first case on 1 March 2020. SARS-CoV-2 genome analysis is an important tool for effective real-time quantitative PCR (RT-qPCR) diagnostics, epidemiology monitoring, as well as vaccination strategy. To date, there is no comprehensive report on the distribution of SARS-CoV-2 genome variants in either the Czech Republic, including Central and Eastern Europe in general, during the first year of pandemic. In this study, we have analysed a representative cohort of SARS-CoV-2 genomes from 229 nasopharyngeal swabs of COVID-19 positive patients collected between March 2020 and February 2021 using validated reference-based sequencing workflow. We document the changing frequency of dominant variants of SARS-CoV-2 (from B.1 -> B.1.1.266 -> B.1.258 -> B.1.1.7) throughout the first year of the pandemic and list specific variants that could impact the diagnostic efficiency RT-qPCR assays. Moreover, our reference-based workflow provided evidence of superinfection in several samples, which may have contributed to one of the highest per capita numbers of COVID-19 cases and deaths during the first year of the pandemic in the Czech Republic.
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