Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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VALUCHOVÁ Soňa MIKULKOVÁ Pavlína PEČINKOVÁ Jana KLIMOVA J. KRUMNIKL M. BAINAR P. HECKMANN S. TOMANCAK P. ŘÍHA Karel

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj elife
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://elifesciences.org/articles/52546
Doi http://dx.doi.org/10.7554/eLife.52546
Klíčová slova OPTICAL MANIPULATION; MOLECULAR-BIOLOGY; MEIOTIC PROPHASE; THALIANA; MEIOSIS; VISUALIZATION; SEGREGATION; EVOLUTION; PROGRAM; TAPETUM
Popis In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.
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