Comparative Electrochemical and Spectroscopic Studies of I-motif forming DNA Nonamers

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

TRNKOVÁ Libuše TŘÍSKOVÁ Iveta VORLÍČKOVÁ Michaela KEJNOVSKÁ Iva DVOŘÁKOVÁ Zuzana PIVOŇKOVÁ Hana FIALA Radovan

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Electroanalysis
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://onlinelibrary.wiley.com/doi/epdf/10.1002/elan.201900323
Doi http://dx.doi.org/10.1002/elan.201900323
Klíčová slova i-motif; (C)-rich DNA nonamers; linear sweep voltammetry; elimination voltammetry with linear scan; CD spectra; NMR spectra; native PAGE
Popis The paper shows the structural diversity of cytosine (C)-rich oligodeoxynucleotides (ODNs) arising from their detail nucleotide sequence and experimental conditions. In slightly acidic solutions, the ODN nonamers with different adenine (A) and cytosine (C) sequences can adopt non-canonical structures involving protonated bases. A distinct secondary structure formed in (C)-rich sequences, called i-motif (iM), consists of hemiprotonated and intercalated cytosine base pairs (C.C+). Folding and unfolding of particular structures in solutions were monitored by H-1 NMR and CD spectroscopies and native polyacrylamide gel electrophoresis (PAGE), which are capable to determine their structural characteristics. Effects of sequences and their proclivity to formation of the iM on electrochemical behaviour of the ODN nonamers were studied by electrochemical methods. The LSV signals of A and C obtained from the reductive dissolution of ODN adsorption layers on a hanging mercury drop electrode were processed by elimination voltammetry with linear scan (EVLS), which revealed complex effects of the nonamer properties (namely their primary and secondary structure confirmed in solution) on their adsorption and reduction activity.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.