PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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PARVANOV Emil Damyanov TIAN Hui BILLINGS Timothy SAXL Ruth L. SPRUCE Catrina AITHAL Rakesh KREJČÍ Lumír PAIGEN Kenneth PETKOV Petko M.

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj Molecular Biology of the Cell
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1091/mbc.E16-09-0686
Obor Genetika a molekulární biologie
Klíčová slova CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION
Popis In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.
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