A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.

Logo poskytovatele

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

GHUFRANA S. PAVLOVÁ Martina ARIF M.I. POSTEMA C.P. DAMBORSKÝ Jiří JANSSEN D.B.

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Applied and Environmental Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1128/AEM.01620-14
Obor Biochemie
Klíčová slova 1; 2;3-Trichloropropane; biodegradation; Pseudomonas putida MC4
Popis 1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment and attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3 dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium P. putida MC4-5222 on TCP was indeed observed, and all organic chlorine was released as chloride. A packed-bed reactor with immobilized cells of strain MC4 5222 degraded >95% of influent TCP (0.33 mM) under continuous flow conditions, with stoichiometric release of inorganic chloride. The results show the use of a laboratory-evolved dehalogenase and genetic engineering for obtaining an effective plasmid-free and stable whole-cell biocatalyst for the aerobic bioremediation of a recalcitrant chlorinated hydrocarbon.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.