Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

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Authors

KOCÁBEK Tomáš ŘEPKOVÁ Jana DUDOVÁ Markéta HOYEROVÁ Klára VRBA Lukáš

Year of publication 2006
Type Article in Periodical
Magazine / Source Genetica
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Arabidopsis thaliana - embryonic defect - mitochondria - pentatricopeptide repeat - semi-lethality - T-DNA mutagenesis
Description A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified among T-DNA insertion lines. Tagging of the At1g53330 gene caused the appearance of a semi-lethal mutation with a complex phenotypic expression from embryo lethality associated with the abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes. The PPR protein At1g53330.1 was predicted to be targeted to mitochondria by TargetP and MitoProt programs. Complementation analysis comfirmed that phenotype is a result of a single T-DNA integration. A thorough functional analysis of this mutant aimed at finding a particular organelle target of At1g53330.1 protein will follow.
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