Molecular portraits of colorectal cancer morphological regions

Investor logo
Investor logo
Investor logo
Investor logo
Investor logo

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

BUDINSKÁ Eva HRIVŇÁKOVÁ Martina CATELA IVKOVIĆ Tina BOUDNÁ Marie NENUTIL Rudolf BENCSIKOVÁ Beatrix AL TUKMACHI Dagmar RUČKOVÁ Michaela DUBSKÁ Lenka SLABÝ Ondřej FEIT Josef MIHNEA-PAUL Dragomir BOŘILOVÁ LINHARTOVÁ Petra TEJPAR Sabine POPOVICI Vlad

Year of publication 2023
Type Article in Periodical
Magazine / Source eLife
MU Faculty or unit

Faculty of Science

Citation
Web https://elifesciences.org/articles/86655
Doi http://dx.doi.org/10.7554/eLife.86655.3
Keywords cancer biology; colorectal cancer; human; intra-tumor heterogeneity; morphology; transcriptomics
Attached files
Description Heterogeneity of colorectal carcinoma (CRC) represents a major hurdle towards personalized medicine. Efforts based on whole tumor profiling demonstrated that the CRC molecular subtypes were associated with specific tumor morphological patterns representing tumor subregions. We hypothesize that whole-tumor molecular descriptors depend on the morphological heterogeneity with significant impact on current molecular predictors. We investigated intra-tumor heterogeneity by morphology-guided transcriptomics to better understand the links between gene expression and tumor morphology represented by six morphological patterns (morphotypes): complex tubular, desmoplastic, mucinous, papillary, serrated, and solid/trabecular. Whole-transcriptome profiling by microarrays of 202 tumor regions (morphotypes, tumor-adjacent normal tissue, supportive stroma, and matched whole tumors) from 111 stage II-IV CRCs identified morphotype-specific gene expression profiles and molecular programs and differences in their cellular buildup. The proportion of cell types (fibroblasts, epithelial and immune cells) and differentiation of epithelial cells were the main drivers of the observed disparities with activation of EMT and TNF-? signaling in contrast to MYC and E2F targets signaling, defining major gradients of changes at molecular level. Several gene expression-based (including single-cell) classifiers, prognostic and predictive signatures were examined to study their behavior across morphotypes. Most exhibited important morphotype-dependent variability within same tumor sections, with regional predictions often contradicting the whole-tumor classification. The results show that morphotype-based tumor sampling allows the detection of molecular features that would otherwise be distilled in whole tumor profile, while maintaining histopathology context for their interpretation. This represents a practical approach at improving the reproducibility of expression profiling and, by consequence, of gene-based classifiers.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.