A comparative assessment of a piezoelectric biosensor based on a new antifouling nanolayer and cultivation methods: Enhancing S. aureus detection in fresh dairy products

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Authors

FORINOVÁ Michala SEIDLOVÁ Anna PILIPENCO Alina SCOTT LYNN JR. Nicholas OBOŘILOVÁ Radka FARKA Zdeněk SKLÁDAL Petr SALÁKOVÁ Alena SPASOVOVÁ Monika HOUSKA Milan KALHOTKA Libor VAISOCHEROVÁ-LÍSALOVÁ Hana

Year of publication 2023
Type Article in Periodical
Magazine / Source Current Research in Biotechnology
MU Faculty or unit

Faculty of Science

Citation
Web https://www.sciencedirect.com/science/article/pii/S2590262823000485
Doi http://dx.doi.org/10.1016/j.crbiot.2023.100166
Keywords Food safety; Biosensor; Antifouling coating; Quartz crystal microbalance; Cultivation-based methods; Detection of Staphylococcus aureus; Dairy products
Description Ensuring dairy product safety demands rapid and precise Staphylococcus aureus (S. aureus) detection. Biosensors show promise, but their performance is often demonstrated in model samples using non-native pathogens and has never been studied towards S. aureus detection in naturally contaminated samples. This study addresses the gap by directly comparing results taken with a novel piezoelectric biosensor, capable of one-step detection, with four conventional cultivation-based methods. Our findings reveal that this biosensor, based on an antifouling nanolayer-coated biochip, exhibits exceptional resistance to biofouling from unprocessed dairy products and is further capable of specific S. aureus detection. Notably, it performed comparably to Petrifilm and Baird-Parker methods but delivered results in only 30 min, bringing a substantial reduction from the 24 h required by cultivation-based techniques. Our study also highlights differences in the performance of cultivation methods when analyzing artificially spiked versus naturally contaminated foods. These findings underline the potential of antifouling biosensors as efficient reliable tools for rapid, cost-effective, point-of-care testing, enhancing fresh dairy product safety and S. aureus detection.
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