Single nanoparticle tag detection in immunoassay by LA ICP MS

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Authors

SVOJANOVSKÝ Vilém MÁČALA Jakub MAŠLAŇOVÁ Ivana FARKA Zdeněk KROUPA Jiří KANICKÝ Viktor HOUŠKA Pavel PREISLER Jan

Year of publication 2023
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Single particle infrared laser ablation (SPIRLA) utilizes desorption of intact nanoparticles from a suitable surface such as nitrocelluse using an IR laser at a wavelenght emmiting at 2940 nm. IR laser ablates nitrocellulose and deposited intact nanoparticles are desorbed. Coupled with ICP MS, nanoparticles can be counted with the single particle detection limit. Enzyme-linked immunosorbent assay (ELISA) with labeling based on the principle of antibody-antigen immunocomplex formation is nowadays a common analytical method. The signal corresponding to immunocomplex formation is usually amplified by an enzyme. In this work, a model assay of the HSA protein (Human Serum Albumin) will be conducted, the enzyme will be replaced by an yttrium upconversion nanoparticle (PLISA, Particle-linked immunosorbent assay). Here we test the possibility of the single particle label detection, furthermore the reaction will be transfered to a nitrocellulose surface and compared to the alternative ULISA (upnconversion particle-linked immunosorbent assay ) detection.
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