Telomerase Interaction Partners-Insight from Plants

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Authors

FULNEČKOVÁ Jana DOKLÁDAL Ladislav KOLÁŘOVÁ Karolína NEŠPOR DADEJOVÁ Martina PROCHAZKOVA Klara GOMELSKÁ Sabina SIVČÁK Martin ADAMUSOVÁ Kateřina LYČKA Martin PEŠKA Vratislav DVOŘÁČKOVÁ Martina SÝKOROVÁ Eva

Year of publication 2022
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://doi.org/10.3390/ijms23010368
Doi http://dx.doi.org/10.3390/ijms23010368
Keywords protein-protein interaction; replication; mitochondria; chromatin; transport; folding; telomerase; Arabidopsis
Description Telomerase, an essential enzyme that maintains chromosome ends, is important for genome integrity and organism development. Various hypotheses have been proposed in human, ciliate and yeast systems to explain the coordination of telomerase holoenzyme assembly and the timing of telomerase performance at telomeres during DNA replication or repair. However, a general model is still unclear, especially pathways connecting telomerase with proposed non-telomeric functions. To strengthen our understanding of telomerase function during its intracellular life, we report on interactions of several groups of proteins with the Arabidopsis telomerase protein subunit (AtTERT) and/or a component of telomerase holoenzyme, POT1a protein. Among these are the nucleosome assembly proteins (NAP) and the minichromosome maintenance (MCM) system, which reveal new insights into the telomerase interaction network with links to telomere chromatin assembly and replication. A targeted investigation of 176 candidate proteins demonstrated numerous interactions with nucleolar, transport and ribosomal proteins, as well as molecular chaperones, shedding light on interactions during telomerase biogenesis. We further identified protein domains responsible for binding and analyzed the subcellular localization of these interactions. Moreover, additional interaction networks of NAP proteins and the DOMINO1 protein were identified. Our data support an image of functional telomerase contacts with multiprotein complexes including chromatin remodeling and cell differentiation pathways.
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