p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice

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Authors

BORA Pablo GAHUROVÁ Lenka MAŠEK Tomáš HAUSEROVÁ Andrea POTĚŠIL David JANSOVÁ Denisa SUSOR Andrej ZDRÁHAL Zbyněk AJDUK Anna POSPÍŠEK Martin BRUCE Alexander W.

Year of publication 2021
Type Article in Periodical
Magazine / Source Communications Biology
MU Faculty or unit

Central European Institute of Technology

Citation
Web fulltext
Doi http://dx.doi.org/10.1038/s42003-021-02290-z
Keywords ACTIVATED PROTEIN-KINASE; INNER CELL MASS; MAP KINASE; PREIMPLANTATION EMBRYO; MESSENGER-RNA; NA/K-ATPASE; 1A MYBBP1A; P38 MAPK; MOUSE; GROWTH
Description Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation.
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