Comparison of Catalysis by Haloalkane Dehalogenases in Aqueous Solutions of a Deep Eutectic and Organic Solvents.

Investor logo
Investor logo

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

ŠTĚPÁNKOVÁ Veronika VAŇÁČEK Pavel DAMBORSKÝ Jiří CHALOUPKOVÁ Radka

Year of publication 2014
Type Article in Periodical
Magazine / Source Green Chemistry
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1039/c4gc00117f
Field Biochemistry
Keywords Haloalkane dehalogenases; DbjA; DhaA; LinB
Description Haloalkane dehalogenases catalyze the hydrolytic cleavage of carbon-halogen bonds in diverse halogenated hydrocarbons and are attractive catalysts for sustainable biotechnologies. However, their use in industrial processes is limited due to the poor water solubility of their substrates and the tendency of the substrates to undergo abiotic hydrolysis. Here we systematically and critically compare the performance of three haloalkane dehalogenases, DbjA, DhaA and LinB, in aqueous solutions of the deep eutectic solvent ethaline, its components (ethylene glycol and choline chloride), and two organic solvents (methanol and acetone). Each of the solvents had different effects on the activity of each enzyme. Haloalkane dehalogenase DhaA was found to be the most tolerant of ethaline, retaining 21% of its reference activity even in solutions containing 90% (v/v) of ethaline. However, dissolution in 75% (v/v) ethylene glycol, 50% (v/v) methanol, or 25% (v/v) acetone caused almost total loss of DhaA activity. In contrast, the activities of DbjA and LinB were higher in ethylene glycol than in ethaline, and the initial activity of DbjA was 1.5 times higher in 50% (v/v) ethylene glycol than in pure buffer. Interestingly, the enantioselectivity of 2-bromopentane hydrolysis catalysed by DbjA increased more than 4-fold in the presence of ethaline or ethylene glycol. Our results demonstrate that ethylene glycol and an ethylene glycol-based deep eutectic solvent can have beneficial effects on catalysis by haloalkane dehalogenases, broadening their usability in “green” biotechnologies.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.