Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2018127
- Project Period
- 1/2020 - 12/2022
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
-
Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
- Cooperating Organization
-
Institute of Biotechnology CAS
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies, will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB is a part of the ESFRI project INSTRUCT-ERIC.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 328
2020
-
Choice of Force Field for Proteins Containing Structured and Intrinsically Disordered Regions
Biophysical Journal, year: 2020, volume: 118, edition: 7, DOI
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Integrated Proteo-Transcriptomic Analyses Reveal Insights into Regulation of Pollen Development Stages and Dynamics of Cellular Response to Apple Fruit Crinkle Viroid (AFCVd)-Infection in Nicotiana tabacum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, year: 2020, volume: 21, edition: 22, DOI
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Introduction to Raman Microscope Renishaw inVia
Year: 2020, type: Workshop
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Isoforms of Cathepsin B1 in Neurotropic Schistosomula of Trichobilharzia regenti Differ in Substrate Preferences and a Highly Expressed Catalytically Inactive Paralog Binds Cystatin
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, year: 2020, volume: 10, edition: FEB, DOI
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KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling
JOURNAL OF CELL BIOLOGY, year: 2020, volume: 219, edition: 6, DOI
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Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
PLOS ONE, year: 2020, volume: 15, edition: 6, DOI
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Molecular insights into the architecture of the human SMC5/6 complex
Journal of Molecular Biology, year: 2020, volume: 432, edition: 13, DOI
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Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk
Journal of Dairy Science, year: 2020, volume: 103, edition: 6, DOI
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Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery
Redox Biology, year: 2020, volume: 32, edition: MAY 2020, DOI
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Octanuclear nickel phosphonate core forming extended and molecular structures
CrystEngComm, year: 2020, volume: 22, edition: 41, DOI