Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2018127
- Project Period
- 1/2020 - 12/2022
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
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Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
- Cooperating Organization
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Institute of Biotechnology CAS
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies, will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB is a part of the ESFRI project INSTRUCT-ERIC.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 327
2022
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Codanin1 and CDIN1 interaction: implications for inherited anemia progression
Year: 2022, type: Appeared in Conference without Proceedings
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Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor
Scientific Reports, year: 2022, volume: 12, edition: 1, DOI
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Comparative analysis of transcriptomic points-of-departure (tPODs) and apical responses in embryo-larval fathead minnows exposed to fluoxetine
ENVIRONMENTAL POLLUTION, year: 2022, volume: 295, edition: FEB, DOI
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Competitor hydrogen-bond acceptors in the SP(NH)3-based structures: Comparison of structural features – Computational/database and experimental
Polyhedron, year: 2022, volume: 228, edition: December, DOI
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Computer-aided engineering of staphylokinase toward enhanced affinity and selectivity for plasmin
Computational and Structural Biotechnology Journal, year: 2022, volume: 20, edition: May 2022, DOI
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Convergent views on disordered protein dynamics from NMR and computational approaches
BIOPHYSICAL JOURNAL, year: 2022, volume: 121, edition: 20, DOI
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Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly
Nucleic acids research, year: 2022, volume: 50, edition: 10, DOI
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Critical interactions of neuronal transcription factor REST with stabilizer TRF2
Year: 2022, type: Conference abstract
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Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion
Communications Biology, year: 2022, volume: 5, edition: 1, DOI
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Describing 14-3-3 dimerization: tools to obtain KD
Year: 2022, type: Conference abstract