Impact of Chromate and Dichromate on Lysozyme Stability: A Spectroscopic and Molecular Docking Investigation

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři

SUBADINI Suchismita PANIGRAHY Ram Sankar GUPTA Nishesh Kumar BERA Krishnendu SAHOO Harekrushna

Rok publikování 2022
Druh Článek v odborném periodiku
Časopis / Zdroj Luminescence
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/bio.4231
Doi http://dx.doi.org/10.1002/bio.4231
Klíčová slova Lyz; fluorescence quenching; anisotropy; chromate; dichromate
Popis A comparative study of interaction between Lysozyme (Lyz) with two hexavalent chromate ions; chromate and dichromate; which are prevalently known for their toxicity, was investigated using different spectroscopic techniques along with molecular docking study. Both steady-state and time-resolved studies revealed that the addition of chromate/dichromate is responsible for strong quenching of intrinsic fluorescence in Lyz and the quenching is caused by both static and dynamic quenching mechanism. Different binding and thermodynamic parameters were also calculated at different temperatures from the intrinsic fluorescence of Lyz. The conformational change in Lyz and thermodynamic parameters obtained during the course of interaction with chromate/dichromate were well-supported by the molecular docking results.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.