Capsid opening enables genome release of iflaviruses.

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Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

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ŠKUBNÍK Karel SUKENÍK Lukáš BUCHTA David FÜZIK Tibor PROCHÁZKOVÁ Michaela MORAVCOVÁ Jana ŠMERDOVÁ Lenka PŘIDAL Antonín VÁCHA Robert PLEVKA Pavel

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Science advances
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.science.org/doi/pdf/10.1126/sciadv.abd7130
Doi http://dx.doi.org/10.1126/sciadv.abd7130
Klíčová slova DEFORMED WING VIRUSBEE PARALYSIS VIRUSCRYO-EMMOLECULAR-DYNAMICSFORCE-FIELDX-RAYPOLIOVIRUSRNAMODELTRANSITIONS
Popis The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.
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