BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana

Varování

Publikace nespadá pod Ekonomicko-správní fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	KITAKURA S. ADAMOWSKI M. MATSUURA Y. SANTUARI L. KOUNO H. ARIMA K. HARDTKE C.S. FRIML Jiří KAKIMOTO T. TANAKA H.
Rok publikování	2017
Druh	Článek v odborném periodiku
Časopis / Zdroj	Plant and Cell Physiology
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	https://academic.oup.com/pcp/article-abstract/58/10/1801/4086060?redirectedFrom=fulltext
Doi	http://dx.doi.org/10.1093/pcp/pcx118
Klíčová slova	Arabidopsis; ARF GEF; Auxin; Brefeldin A; PIN-FORMED1; trans-Golgi network
Popis	Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to the fungal toxin brefeldin A (BFA), which is known to inhibit guanine nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been fully revealed. In a previous study, we identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. BEN3/BIG2 tagged with fluorescent proteins co-localized with markers for the TGN/early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA sensitive, and established BEN3/BIG2 as a crucial component of this BFA action at the level of the TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF, BEN1/MIN7. Taken together, our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
Související projekty:	CEITEC - středoevropský technologický institut Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách